Released on = June 14, 2007, 1:31 am

Press Release Author = Jessica Castro

Industry = Biotech

Press Release Summary = With the industry growing so rapidly, there are "young"
companies sprouting all over the place. Many of these same companies are not
successful and do not last very long.

Press Release Body = With the industry growing so rapidly, there are "young"
companies sprouting all over the place. Many of these same companies are not
successful and do not last very long. Bio-Synthesis has been the world leader in
custom services since our inception in 1984. Over two decades of the highest quality
of products, coupled with fast turnaround time and affordability has enabled BSI to
stand in no one else's shadow. Go with a company you know you can trust. Go with a
company who cares about your research needs as much as you do. Whether its peptides,
antibodies, DNA, RNA, or PNA, BSI will treat your needs like our own. Contact us now
for your next quote, and see the difference a well-established company can make.

1.Synthesis of Novel Backbone Modified DNA

Bio-Synthesis inc., senior scientists Dr\'s Jianhua Ye and Miguel Castro completed
the synthesis of a new kind of modified DNA. Normally there is a phosphodiester
backbone linking the nuecleotides in a DNA strand, however hese diester bonds are
susceptible to degradation by DNA\'ses, present in living cells. One way to extend
the half life of DNA is by modifying the phosphodiester backbone. In this work four
monomers were constructed, which upon chemical coupling produce a peptide like
backbone which is unaffected by nucleases, thereby increasing the half life of these
molecules and consequently extending its intended activity.

2.The DNA Synthesis Division of Bio-Synthesis Inc is happy to announce another
custom service: Mini Gene synthesis; with the capability to synthesize 100-200 bases
long oligonucleotides we can put together small genes together; a small gene can be
defined as one that encodes for 30-60 residuesin length. The ability to chemically
synthesize proves very useful to the biomedical researcher that studies control of
gene expression; a minigene can code for a peptide that can be an antagonist to a
regulatory protein. We can deliver minigenes in about 5-7 days. For more information
please call the DNA Synthesis Division.

3.Bio-Synthesis Inc is proud to announce the recent acquisition of a sophisticated
Multiple Peptide Synthesizer, the Vantage from ACT, Louiville, Kentucky. This
instrument has four dispensor tips, which allows for parallel washings, decreasing
cycling times for peptide assembly. The Vantage is also equipped with a heating
device which allows use to conduct reactions at 80 degrees Celsius, which expedites
coupling times. This unit will help us to prepare a large number of peptides( 96 at
a time) for applications such us peptide microarrays and discovery scale peptides;
where researcher needs small quantities(imilligram or less) but in large numbers.

Bio-Synthesis has released an updated description for peptide synthesis; this allows
peptide researchers to get a view of the broad spectrum of our capabilities:
Peptides are complex molecules and each peptide sequence is unique with regard to
its chemical and physical properties. Peptides are synthesized by the solid-phase
method using Fmoc chemistry on an Omega 396 multiple peptide synthesizer from
Advanced ChemTech. The Core also uses an Applied Biosystems Model 431A Synthesizer
for longer peptides. Synthesis is carried out at 50 µmole, 100 µmole or 250 µmole
scales. On an average, a synthesis scale of 100 µmoles will yield about 50-70 mg of
a 10-residue peptide and 100-150 mg of a 20-residue peptide depending on the
substitution rating of the resin. The peptide synthesis service provides a complete
spectrum of services that include, synthesis, cleavage, HPLC purification, peptide
modifications, cyclization and mass analysis for quality assurance. Before
submitting your peptide sequences to the Core, it is advisable to consider the
following:

* Every peptide sequence synthesized is a unique one. Some peptides sequences are
difficult to synthesize although, we attempt to synthesize every peptide sequence.
* Peptides with multiple cysteines, methionine, arginine and tryptophan residues can
often be difficult to synthesize.
* Avoid a proline residue at the C-terminal of the peptide.
* The peptide will be de-protected and cleaved from the resin with 82.5-90%
trifluoroacetic acid, partially purified, and lyophilized. The lyophilized material
will be provided as a \"crude\" preparation. Turnaround time is about ten days to
three weeks depending on the length of the peptide and purity level. When requested,
the peptide will be purified further (see below).

For further questions please feel free to contact our Peptide Synthesis Division at
1800-227-0627
4.Peptide-PNA bioconjugates

Our peptide Synthesis Division has successfully Developer methods to synthesize
bioconjugates such us PNA's linked to peptides; the hybrid molecule can be up to 60
residues in length and can be half PNA and half peptide linked together or they can
be alternating PNA residues with peptide residues. This type of bioconjugates can be
used to deliver peptide sequences inside a living cell, by using peptide sequences
at one end that have the ability to penetrate the cell membrane and carry along a
PNA, that can be targeted to a regulatory DNA sequence, then upon binding to it, it
can interfere with its expression( production of a protein).

5.We are happy to announce that Biosearch Technologies of Novato, California, have
extended Bio-Synthesis Inc, of Lewisville, Texas their line of Fluorescent dyes and
quenchers for use in biomedical research applications. End users who source
Biosearch dyes for any purpose other than non-commercial R&D applications will still
be required to contact the Biosearch Technologies Licensing Department to obtain a
Commercial Use License. These dyes are chemically couple to synthetic
oligonucleotides and provide an excellent means for non-radioactive detection of DNA
targets. Bio-Synthesis Inc have been providing custom synthetic oligonucleotides to
the biomedical research community worldwide since 1984.





Web Site = http://www.biosyn.com

Contact Details = Jessica Castro
Biosyn